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别名
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AEC, 50X ;3-氨基-N-乙基咔唑;AEC, 50X
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产品介绍
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AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
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用途
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过氧化物酶底物.Usage? Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps.? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;).? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well.? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT.? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain.? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; )? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations
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生化机理
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3-氨基-9-乙基咔唑是一种过氧化酶底物，适用于免疫印迹和免疫组织化学染色程序。该底物能产生一种不溶性红色的末端产物。肉眼或可见光下就可观察。
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氢氧化铜，CP，95%
3,4-二甲氧基苯甲酸 9...
S-碘化丁酰硫代胆碱 98%
S-联萘酚 99%
α,α,α-三氟苯乙酮 99...
苯基三乙氧基硅烷 ,98%
苯甲醇 ,AR,≥99%
正辛酸 CP,98%
辛酸钠 99%
氯化镁，AR，98%
2-氨基-3-(三氟甲基)吡...
α-松油醇 ，GC≥96%
苯丁酸，AR,97%
4-硝基吲唑，97%
4-氯咪唑，97%
聚(4-苯乙烯磺酸钠)，av...
2-溴苯甲胺，98%
N-羟基正辛酰胺，98%
2,2 -双噻吩，98%
4,4 -二溴二苯甲酮，97...
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