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别名
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双(3-羧基-4-硝基苯基)二硫，6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 ;DTNB,Ellman’s Reagent;二硫代硝基苯甲酸;3-Carboxy-4-nitrophenyl disulfide;6,6‘-Dinitro-3,3‘-dithiodibenzoic acid;Bis(3-carboxy-4-nitrophenyl) disulfide;DTNB; Ellman’s Reagent;DTNBEllman’s Reagent
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产品介绍
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Ellman‘s Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.Ellman‘s Reagent (5,5‘-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman‘s Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.Highlights:?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm)?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
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用途
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巯基测定，是测定蛋白质、多肽和组织中巯基的灵敏试剂。也用于有机磷农药中毒的监测（胆碱酯酶测定）。
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021-54720761
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