核糖核酸酶T1,BR,1000u/ul-elisa检测试剂盒-酶联免疫试剂盒-elisa kit供应商-上海晶抗生物工程有限公司

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核糖核酸酶T1,BR,1000u/ul
货号：G63249 CAS号：9026-12-4

英文名：Rnase T1

产品订购

产品货号	重量	货期	优惠价	数量
G63249-100KU	100KU	准现货	700.00
G63249-500KU	500KU	准现货	2500.00

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货号：G63249
CAS号：9026-12-4
分子式：
分子量：
Beilstein号：
EC号：232-809-1
MDL号：MFCD00132191
PubChem编号：

属性

存贮条件	储存温度 2-8°C
密度	否
荧光	否
IC50	否
pK Values	否

描述

别名	RNase T1;Guanyloribonuclease;Ribonucleate 3‘-guanylo-oligonucleotidohydrolase
产品介绍	Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3‘-phosphate residues (or inosine 3‘-phosphate) and the 5‘-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2‘, 3‘-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3‘-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
用途	Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
生化机理	Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2’:3’ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3’-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3‘-GMP and oligonucleotides with a 3‘-GMP terminal group.

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