别名
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荧光素酶;重组型酵素;荧光素酶(萤火虫);北美产萤火虫(萤火虫);RFLuc;Luciola mingrelica luciferase; Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); Photinus pyralis luciferase; Pyrocoelia pygidialis luciferase; Pyrophorus plagiophthalamus luciferase; RFLuc; Ultra-Glo; Ultra-Glo luciferase
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产品介绍
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Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light.
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用途
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Luciferase from Photinus pyralis has been used in a study to identify the different characteristics of reporter genes in whole-cell bacterial sensors. Luciferase from Photinus pyralis has also been used in a study to develop a novel bioluminogenic assay for alpha-chymotrypsin.The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. Less than or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
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生化机理
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Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
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