蛋白酶S，BR，500u/mg-elisa检测试剂盒-酶联免疫试剂盒-elisa kit供应商-上海晶抗生物工程有限公司

试剂
酶类

蛋白酶S，BR，500u/mg
货号：G63391 CAS号：66676-43-5

英文名：V8 Protease

产品订购

产品货号	重量	货期	优惠价	数量
G63391-1mg	1mg	准现货	1300.00
G63391-5mg	5mg	准现货	4500.00

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货号：G63391
CAS号：66676-43-5
分子式：
分子量：
Beilstein号：
EC号：
MDL号：MFCD01324998
PubChem编号：

属性

存贮条件	储存温度 2-8°C
密度	否
荧光	否
IC50	否
pK Values	否

描述

别名	蛋白内切酶 Glu-C 来源于金黄色葡萄球菌 V8;Endoproteinase Glu-C from Staphylococcus aureus V8;Protease,Staph aureus from Staphylococcus aureus V8
产品介绍	Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH‘s of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
用途	Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine protease used for selective cleavage of proteins for amino acid sequence determination or peptide mapping .
生化机理	Staphylococcus strain V8 protease specifically cleaves peptide bonds on the carboxyl side of aspartic and glutamic acid residues when used in phosphate buffer. When used in ammonium bicarbonate buffer or ammonium acetate buffer cleavage is restricted to the carboxyl side of glutamic acid residues only. The enzyme exhibits maximal activity from pH 4.0 to 7.8. If hemoglobin is used as the substrate, maximal activity is at pH 4.0. The maximal activity is at pH of 7.8 when casein is the substrate.
备注	Chromatographically purified according to Drapeau, G., Boily, Y., and Houmard, J.: JBC, 247, 6720 (1972). A lyophilized powder.

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